MMP13 monoclonal antibody (M07), clone 3B11
产品名称: MMP13 monoclonal antibody (M07), clone 3B11
英文名称: MMP13 monoclonal antibody (M07), clone 3B11
产品编号: H00004322-M07
产品价格: null
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围:
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant MMP13.
- Immunogen:
- MMP13 (NP_002418, 362 a.a. ~ 471 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- ILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
- MSDS:
- Download
- Applications
- Western Blot (Transfected lysate)
- Western Blot analysis of MMP13 expression in transfected 293T cell line by MMP13 monoclonal antibody (M07), clone 3B11.
Lane 1: MMP13 transfected lysate(53.8 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of MMP13 transfected lysate using anti-MMP13 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MMP13 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged MMP13 is approximately 1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of MMP13 over-expressed 293 cell line, cotransfected with MMP13 Validated Chimera RNAi ( Cat # H00004322-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MMP13 monoclonal antibody (M07), clone 3B11 (Cat # H00004322-M07 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 4322
- GeneBank Accession#:
- NM_002427
- Protein Accession#:
- NP_002418
- Gene Name:
- MMP13
- Gene Alias:
- CLG3
- Gene Description:
- matrix metallopeptidase 13 (collagenase 3)
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq
- Other Designations:
- collagenase 3,matrix metalloproteinase 13,matrix metalloproteinase 13 (collagenase 3)
- Related Disease
- Adenocarcinoma
- Alveolar Bone Loss
- Aortic Aneurysm, Abdominal
- Arteriosclerosis
- Arthritis, Rheumatoid
- Atherosclerosis
- Breast cancer
- Breast Neoplasms
- Carcinoma, Squamous Cell
- Cardiovascular Diseases
- Chorioamnionitis
- Chronic Periodontitis
- Cleft Lip
- Cleft Palate
- Coronary Artery Disease
- Diabetes Mellitus, Type 2
- Disease Progression
- Edema
- Endometriosis