Syn1 (phospho S549) polyclonal antibody
产品名称： Syn1 (phospho S549) polyclonal antibody
英文名称： Syn1 (phospho S549) polyclonal antibody
产品编号： PAB9656
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Syn1.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding S549 of rat Syn1.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 78
- Reactivity:
- Bovine, Dog, Human, Mouse, Primates, Rat
- Form:
- Liquid
- Purification:
- Affinity purification
- Storage Buffer:
- In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Regulation of neurotransmitter release by synapsin III.
Feng J, Chi P, Blanpied TA, Xu Y, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P.J Neurosci. 2002 Jun 1;22(11):4372-80.
- 2.
- A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth.
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P.Nat Neurosci. 2002 May;5(5):431-7.
- 3.
- Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals.
Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC Jr, Greengard P, Czernik AJ.J Neurosci. 2001 Oct 15;21(20):7944-53.
- Applications
- Western Blot (Tissue lysate)
- Western blot of rat cortex lysate showing specific immunolabeling of the ~78k Syn1 phosphorylated at Ser549 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase, lane 2). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Syn1 (phospho S549) polyclonal antibody (Cat # PAB9656). The immunolabeling is completely eliminated by treatment with lambda-Ptase.