SMAD3 polyclonal antibody-抗体-抗体-生物在线
亚诺法生技股份有限公司（Abnova)
SMAD3 polyclonal antibody

SMAD3 polyclonal antibody

商家询价

产品名称： SMAD3 polyclonal antibody

英文名称： SMAD3 polyclonal antibody

产品编号： PAB11263

产品价格： null

产品产地： 台湾

品牌商标： Abnova

更新时间： null

使用范围：

亚诺法生技股份有限公司（Abnova)
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SMAD3.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 417-425 of human SMAD3.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Clawed frog, Frog, Human, Mouse, Pig, Rat, Zebra fish
  • Specificity:
  • ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human Smad3. Expect reactivity against Smad1 and Smad5. Negligible reactivity is seen against other Smad family members.
  • Form:
  • Liquid
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:500-1:3000)
    Immunohistochemistry (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using SMAD3 polyclonal antibody (Cat # PAB11263) shows detection of endogenous SMAD3 in both unstimulated and stimulated cell lysates.
    Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/mL TGF-beta (+ lanes) for 1 hour.
    This reagent recognizes both non-phosphorylated and phosphorylated SMAD3 protein.
    Personal Communication, Ying Zhang, NIH, CCR, Bethesda, MD.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining with SMAD3 polyclonal antibody (Cat # PAB11263) shows strong cytoplasmic and membranous staining of tumor cells in cancerous human liver tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.
    Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4088
  • Protein Accession#:
  • NP_005893;P84022
  • Gene Name:
  • SMAD3
  • Gene Alias:
  • DKFZp586N0721,DKFZp686J10186,HSPC193,HsT17436,JV15-2,MADH3,MGC60396
  • Gene Description:
  • SMAD family member 3
  • Gene Summary:
  • The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
  • Other Designations:
  • MAD, mothers against decapentaplegic homolog 3,SMA- and MAD-related protein 3,SMAD, mothers against DPP homolog 3,mad homolog JV15-2,mad protein homolog,mothers against decapentaplegic homolog 3

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